FastQCFastQC Report
Thu 23 Jun 2016
MG394536Y_S20_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394536Y_S20_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588683
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1200.001579083938.1752059
CGCCGCA1800.001713251337.543736235
GGGTAGG1400.003363866432.7216031
CCTGTAC5001.8189894E-1229.7766593
GGACCGT1550.005535203529.5549986
GGACCAC1650.00751184627.7637846
CCTATAC3957.835479E-826.0944423
GTATAAT9850.025.5793251
ACCGTGC2350.001423380524.3671538
ATAGAGT4502.762663E-722.905126
CAGTACT4653.7894824E-722.1662484
TATAGGA5255.0466042E-821.8144042
GTCTTAG5305.5790224E-821.6086061
AGAGGGT3304.1618105E-420.8228387
TATACCG4405.814203E-620.8228385
TAGACTG3455.6164904E-419.9174965
TCTATAG3506.188089E-419.6329632
CGTATAC5901.7302955E-719.4111213
GTAGTAC2950.00532641919.4111213
ACCGTGT3000.005869675419.08768