FastQCFastQC Report
Thu 23 Jun 2016
MG394535H_S19_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394535H_S19_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846332
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT4059.640644E-1131.0414351
GTATATG9850.024.3661671
CCTAGGA2901.7640718E-423.6417942
CTATACT8950.022.981414
CCTATAC6003.7107384E-1022.8537353
TATACTG9650.021.3143635
CTATAGC4355.3744716E-621.0149274
CCTATAT7151.4733814E-1020.7761233
CTATACG6051.015178E-820.7761214
TCCTATA6601.2205419E-920.7761212
AGGCCGT2800.00399122420.4051196
GTCCACA4659.552681E-619.6626111
GTGTGGG2950.005392404719.3710061
CTAGGAC2950.00539793319.367573
CGCACGA4600.006274550718.868204235
TATAGTG7304.278263E-918.7838925
GTATAAG10051.8189894E-1218.1952511
GTGTAGA7556.4810592E-918.1651271
ACCGTGC3150.00788274218.1378848
ACTATAC7005.4131306E-817.9565053