FastQCFastQC Report
Thu 23 Jun 2016
MG394535H_S19_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394535H_S19_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846332
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGT3851.9190338E-929.66684
GTATATA8850.023.2306121
CTTACAG7003.6379788E-1222.8434373
CAGACCC2600.002603871721.9648424
GTATTAG16600.021.3297141
CTTATAC10350.020.9674053
CCTCTAG3905.5379132E-520.500521
GGACTCA2850.004433418620.0381036
CCTTACA6552.5418558E-819.181512
TATAGGT7754.3655746E-1019.159012
GTCTTAG6602.7734131E-819.0361961
CAGTGCA5501.9505642E-618.6900849
GTATAGG4901.5088675E-518.6477031
ATATACC8801.2187229E-1018.1709163
TATACTA8801.2187229E-1018.1709165
CTATACC5702.744091E-618.0342924
ACACTGT8251.0040822E-917.9978586
TAGAGTG3900.00130434517.5718755
CATATAC11050.017.5718753
ATAAGGG8001.3367753E-817.1325783