FastQCFastQC Report
Thu 23 Jun 2016
MG394206S_S17_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394206S_S17_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956206
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGCC1250.00190062136.7637753
CCTTAGG2500.002002395522.9809651
GTCTAGT7651.4551915E-1121.0283341
ACAGGGC3354.5122762E-420.576743
GCCGTGT2900.004740157619.8080656
TATAGAG8304.9112714E-1119.3784942
CTACGCT4851.313641E-518.95044
TACACTG8608.0035534E-1118.7025015
GTATAGA9251.0913936E-1118.6332131
CCCACAC4401.3373868E-418.2774453
ACAGTGC5102.0251893E-518.0214588
TAGGACC3200.00836823917.9510614
CTACTCT6504.638714E-717.674894
CTACACT7257.6035576E-817.4310994
CTATACT10154.7293724E-1116.9783444
GTATATG11651.8189894E-1216.7672271
GTATTAA13200.016.539331
TATACAG14000.016.41245
GTATTAT19050.016.2857231
TCTGGAC7801.7498132E-716.2019843