FastQCFastQC Report
Thu 23 Jun 2016
MG394206S_S17_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394206S_S17_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956206
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC4356.6320354E-926.4122413
TATACTG14600.024.3951425
TAGGACC4402.1593223E-723.500894
TAGGTCG3452.054869E-523.3116745
TATACCG7105.456968E-1222.6550055
ACTGTGC5254.8863512E-821.8844288
CCTGGGG2700.003132531721.2765273
GTCCTAG4608.315334E-619.9814361
ACTATAC7552.8376235E-1019.7829443
ACCGTCC4751.0970349E-519.3504418
GTATAGG5401.5470214E-619.1488741
CCTAGGT3050.00634201118.8349593
CTATACT11000.018.8007144
GTATACT11150.018.5477894
CTGTGCG3759.6376013E-418.3829219
GTCCTAT5152.2013845E-517.8474961
CGTATAC9702.3646862E-1117.7669983
TAGCCTG3250.0091470817.6758845
CCAGGAC3950.001365679317.4521393
TAGGTTG5953.907604E-617.378815