FastQCFastQC Report
Thu 23 Jun 2016
MG394203X_S18_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394203X_S18_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812405
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG4601.2732926E-1129.7296541
TAAGGGT2753.7680365E-629.0053654
CCTAGGT2250.001138040825.3221453
TATAGGA6202.1827873E-1123.8926682
TAGGACC3401.9558782E-523.4602224
AAGGGTG3603.0421586E-522.1568785
TATATAG8250.022.0993295
TGTGTGC3153.14638E-421.7046958
GTATAGA7551.4551915E-1121.1323381
GTAAGGG3304.3077907E-420.7181193
TATAGTG6651.3878889E-920.5623425
AGGGTGA2900.004969481419.6464926
CTCTGGG2900.004969481419.6464922
CTTATAG6402.0010702E-819.5850963
TATAGCG7304.42742E-918.731455
GTCTATG5501.9922554E-618.6486021
TAGACAG7405.2423275E-918.4783235
GTATAGG4951.678435E-518.4183731
TATATAC10001.8189894E-1218.2319433
GCAGTGT3200.00877150117.8046328