FastQCFastQC Report
Thu 23 Jun 2016
MG394203X_S18_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG394203X_S18_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812405
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT2354.3066102E-529.0891441
TATACCG5650.028.2310565
TACACCG6750.027.0062185
TCTGGGC3109.57277E-625.726693
CTATACC5852.8012437E-1023.3707664
CCTATAC5453.1468517E-922.9955463
CTAGGCA3052.5322102E-422.4129454
CTATAAC7700.022.194643
GTCCTGG3102.8266493E-422.0514491
TATACTG10050.021.5394745
GTATATA11200.021.3623391
GTATAGG5356.5167114E-821.2957921
GGGCTGT4906.5996755E-720.9263736
TACGTAG4906.5996755E-720.9263735
GTGTAAG5651.1593875E-720.1650411
TAGTGTC4006.8588546E-519.9381854
ATACGGT9300.019.6012886
ACCGTGT3506.41114E-419.5312828
GTACTAG5951.999615E-719.1483171
GTCCTAG3658.5021835E-418.7286261