FastQCFastQC Report
Thu 23 Jun 2016
MG393070J_S13_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG393070J_S13_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782094
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGC1450.00387950431.783688
CCTATAC4900.030.5674693
CTAGACT4407.2668627E-926.1854174
CAGGGCA2751.1631632E-425.1389
ATAGGAC3552.507101E-522.7186163
CCTTAAG3052.3472283E-422.6654133
GTTAGCC3253.6063837E-421.2706183
CCCTATA3955.7118345E-520.4179972
GTGCTAC4557.3695555E-620.2616161
AGTGCTC3505.945071E-419.7512878
GTATTAG14850.019.4003281
GTATAGA7203.245077E-919.2063261
CTTATAC8557.094059E-1118.8657513
GTGTATA7354.1909516E-918.8143581
GGCTAAC3100.00684558118.5867651
ATACTGC9301.2732926E-1118.58326
CGTGTAC3759.4557175E-418.4345343
CCTACAC6303.2411845E-718.2882273
ACTAGGG3150.007515839318.2882273
ATCTAAG10750.018.2236751