FastQCFastQC Report
Thu 23 Jun 2016
MG393070J_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG393070J_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782094
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT5701.7644197E-1024.2525484
GTATAGC7700.022.4414831
GTATAGG4653.5843368E-722.2966981
CACCGTC5007.204944E-720.7359287
GCCTTAC3905.1838804E-520.6768531
CACTGTC5057.927538E-720.5306227
GTACACG3505.950717E-419.7485034
ACCGTGG3000.00567565719.1999348
CTATATA7303.8671715E-918.9369224
GACCGTG4301.0978486E-418.7534247
CCCTACA5001.6676997E-518.4319362
CTAATAC15100.018.3098723
CCTGGAG3150.007521948318.2856523
GTGTAGA8901.2551027E-1018.1212861
ACCGTCC3200.0082368317.9999378
GGGTCCA1900.002337642217.867832235
GTACTGT7105.8005753E-817.8478266
GTATATA9101.72804E-1017.7230171
AGCGTCC3250.00900644717.7230158
CTTAGGC3250.00900644717.7230153