FastQCFastQC Report
Thu 23 Jun 2016
MG392174G_S12_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG392174G_S12_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833953
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGG3007.2476287E-626.6656441
ATAGGAC4908.8766683E-1025.6488423
TATACTG10100.024.8869935
TAGCCTG2300.001273687624.837815
TATACAG11050.024.8153325
CCTATAC5101.4133548E-924.6430033
CTTACAC6252.3646862E-1123.7648163
CCTAAGG2901.7655849E-423.6387443
CTATACT8350.023.2612784
ACCGTGC3052.4844645E-422.4761838
CCTGGAC2600.002598981621.9719093
GTATAGG5457.6741344E-820.9690531
CCGTGTA4957.0820715E-720.7734419
GTGTAGA9350.019.5561641
GGCGTAC4108.10004E-519.5114481
ACTATGC6502.3192115E-819.3352818
GTATGTA7153.3014658E-919.1800841
TCCTATA6653.012974E-818.8991452
TATAGTG8006.6393113E-1018.5662632
GTATAAA18600.018.4324741