FastQCFastQC Report
Thu 23 Jun 2016
MG392174G_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG392174G_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833953
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCT2309.151936E-734.772154
TATACTC7300.031.301745
CCGTGCC1600.00654167228.5628389
GTCGTGC2701.087281E-425.3891898
CTATACT8100.023.978684
GTATATA11150.023.5675431
ACTGGCC2500.002069682822.8502713
TATACTG10650.021.4556525
CTAGGAC3203.4378158E-421.4221293
TTATACT11250.021.326924
CCTACAC6701.4752004E-920.4629293
GTATATG10100.020.3616261
GTGTAGA8503.6379788E-1220.1620041
GGTGCAC3405.1756273E-420.1628
TAGGTGG7603.3469405E-1019.5429955
TATACGG7052.7794158E-919.4470395
GCTATAC8254.7293724E-1119.3881073
CTATACC6552.5329427E-819.187254
TATAGGG4209.7661396E-519.0418913
GCACTGA6052.3139728E-718.8845216