FastQCFastQC Report
Thu 23 Jun 2016
MG391826E_S10_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391826E_S10_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782935
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCCT1150.001272055639.893726
GTATAGA7550.027.3496111
ACGGCGC1750.00993889126.2158728
GTGTAGG5503.2305252E-922.9432831
TATACTG9300.022.1989235
TCTATAC7851.8189894E-1221.9161343
GTCTAGA5254.9638402E-821.8507461
GTCTAGG3153.006788E-421.8507441
GTGTAAG5805.9644663E-921.7565631
CTAGGAC3203.3488203E-421.5052073
TAGGACT5558.949428E-820.6656654
CTATACT6851.8517312E-920.0924574
GTATATA10550.019.572471
TAGGATC7354.443791E-918.7256224
GTGTAGT6152.6337693E-718.6530761
CTTAGGT5552.0433527E-618.5990983
GCGTATA5001.7291637E-518.3546261
TAGGACA5652.423656E-618.2699094
TATAGTG6353.6934034E-718.0621175
TATACAG10800.018.0537555