FastQCFastQC Report
Thu 23 Jun 2016
MG391826E_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391826E_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782935
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGC1650.00745794527.805918
GCCGTGC1650.00745794527.805918
TATACTA7300.025.1395915
GTCCTGG2901.7194868E-423.7309071
TCCTATA5452.912202E-923.1503352
CTATACA7700.022.3440384
AGTGTGC3102.7011332E-422.199888
GCTATAC8500.021.5904733
TATACAC8201.8189894E-1220.9815963
TCTATAC7352.0190782E-1020.2869683
GTACTAC2950.005281467519.4405731
TAGCACG3658.126928E-418.8546944
TATACTC6703.13903E-818.8312425
ATATACT13150.018.3170134
TAGACTG4401.3538211E-418.247635
GTGTGGG3150.00771309218.2062511
GTAGTAC3200.00844600517.9217783
CCTATAC4501.6080175E-417.8421273
GTCTTAT10303.6379788E-1217.817381
GTATTAC7758.565621E-917.7599051