FastQCFastQC Report
Thu 23 Jun 2016
MG391787L_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391787L_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705304
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCCG1550.005598391429.4872386
GTGTGAG1700.00878549126.887331
GTCTAAG3007.2505736E-626.6632691
TATACCG3958.016286E-826.0346185
CTATACC4551.1337761E-825.1127574
ACTGTGC2751.2304749E-424.9301198
TAGACGG5053.4297045E-822.6263475
GTATAGA6756.730261E-1122.0077741
GTATATG7651.6370905E-1120.9123691
TCTATAC6101.11595E-820.604813
CTAGGAC2850.004422411320.0461483
CCTACAC4608.71472E-619.8718343
CTACACT5801.481585E-719.7005254
GTATAAC7553.0559022E-1019.6758251
AGAGTGC3506.286166E-419.5879528
TATAGTG5851.621811E-719.5321435
CTATACA7103.0286174E-919.3120654
GTACTAG4751.1489508E-519.2456671
CGTAGAC3607.59832E-419.043843
TATAGGC3658.336728E-418.7829673