FastQCFastQC Report
Thu 23 Jun 2016
MG391787L_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391787L_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705304
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG1550.00559653429.489245
TCAGGCC1600.0065355628.5677013
CCGTGTG2300.001272396924.841489
ACAGGGC2300.001272396924.841488
CAAGACC7055.456968E-1222.6920764
TATACCT5604.1600288E-922.4460534
AGTACCG4254.391368E-621.5097985
GGACGCA2750.00359465520.776516
GTGTAAG5151.0339209E-619.9696561
GTACTAT5201.1343E-619.777641
ACAATGC7103.0267984E-919.3133758
CATATAC8305.2750693E-1119.2745933
GCACCGT3607.5948494E-419.0451346
CTAGTAC4209.750671E-519.0451343
CTAATAC12950.018.5304013
TAAGGTC3100.007186003518.4307753
ACCGTCC3100.007186003518.4307758
GTATATA8751.10958354E-1018.2833291
GTATTAG15050.018.2225861
CAGGGCA3150.00788062318.1382249