FastQCFastQC Report
Thu 23 Jun 2016
MG391785X_S5_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391785X_S5_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982729
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTCG2354.2387743E-529.157847
GGGAGCG2500.002075198822.8403077
TACACTG8450.021.623965
CCTGCAC4456.5779586E-620.5306133
GTCTAGA5801.4906982E-719.6909221
CCCTAGA4108.142325E-519.4988161
TCTACAC9400.019.438563
GTATAGG5901.7847742E-719.3571781
ACTGAGG2950.00541690819.3561923
ATAGGGT4158.940252E-519.262913
ACCCTAT4251.0732766E-418.8096666
TATACTA10400.018.6675595
ACCGCAC3100.00721315218.4196038
GTATAAT20500.018.3845981
GTATATG12450.018.3465631
TAGAGCG4501.6629903E-417.7646835
GTGTATG7759.042196E-917.6837181
ACTGGGC3900.001305746517.5694688
TACCGAG5302.967364E-517.2379677
GTACATA10807.2759576E-1216.9196071