FastQCFastQC Report
Thu 23 Jun 2016
MG391785X_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391785X_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982729
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC6150.027.8432943
TATACCG8200.025.0589645
TAGAGAG5651.7644197E-1024.2458425
GTCCTAG4052.917679E-622.549631
CACCGTG4153.6054898E-622.0062667
CCTACAC6909.276846E-1121.5079353
TAGACAG7902.5465852E-1120.2304445
TATATAC14800.020.0546973
CCTATAG5151.0446638E-619.9498541
TTATACT15250.019.4629174
GTGTAGG6602.791603E-819.026251
TTATACC10900.018.8516964
GGACACC3658.391884E-418.7656156
AGTGGGC3050.00658176218.7143448
CTAAGGG3050.00658176218.7143443
ACACTGT8659.640644E-1118.4763576
AGTACTG8057.2941475E-1018.4353735
ATTATAC20950.017.981853
TACACAG5752.9986022E-617.8681325
TACACTG8351.1878001E-917.7730245