FastQCFastQC Report
Thu 23 Jun 2016
MG391778Z_S6_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391778Z_S6_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904890
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGG4153.4611912E-622.1097552
GTATATA12550.021.9384291
TACACCG8800.020.8535185
TACTATA10850.020.0847242
TCTACAC9200.019.9468423
TAGGGAC4057.170458E-519.8237155
GTATATG11800.019.4440171
GTATAAC9601.8189894E-1219.119951
CGTACAC4801.21872035E-519.1157253
GTATTAG17750.018.7429371
CTATAGT4901.4558105E-518.7256054
GTACTAG6202.8680552E-718.5031781
GTCTTAG6202.8680552E-718.5031781
CTACACC9951.8189894E-1218.4433124
CTCCGAG3200.00844913317.9209927
CACTCCG3200.00844913317.9209925
TATAGAC7055.6290446E-817.895573
GTACTAC3850.0011616217.8783931
TAGGCTC3850.00116334417.8744415
CTATACT9702.3646862E-1117.7362384