FastQCFastQC Report
Thu 23 Jun 2016
MG391778Z_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391778Z_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904890
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAG5051.8189894E-1229.5194247
TAGACCG3406.113987E-726.9815545
CCTAGGT1750.00994934126.2106533
GTCGGGT1750.00994934126.2106538
GTCCTAG3801.6124195E-624.141391
ACCGAGT2951.9342065E-423.3230388
AGAGTGC5452.921297E-923.1447288
GTATAGG6104.3655746E-1022.558351
CCGAGTA4203.8466787E-621.842219
CACCGTG3153.015243E-421.8422097
TATACTC6857.8216544E-1121.7624935
GTCTTAA7802.0008883E-1120.5820831
TCCCTGG2850.004332925720.1178251
GTCTTAG6401.8619176E-819.709181
CTTATAC10150.019.2060813
TTATACC11350.019.1961294
CTATACT9059.094947E-1219.0063444
CAGTGCA6652.8901923E-818.9682359
TATACCG5451.7210186E-618.9365965
CCTATAC6152.6457565E-718.6457883