FastQCFastQC Report
Thu 23 Jun 2016
MG391777G_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391777G_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832799
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGA1802.922693E-431.946939
AGCGTCG2158.269698E-426.7462658
TATACCG5551.2914825E-1024.8667985
CCTATAC5151.4661055E-924.5650183
GTATAGG4952.5858753E-823.2383161
TAGACTG6053.8016879E-1022.8116915
GTATAGC7155.456968E-1222.523291
CTAGAGT4153.3793676E-622.17044
CTATACT7501.0913936E-1121.4683384
CGTATAC7101.2369128E-1021.0579763
CCCTATA3304.047118E-420.9107172
ACAGCGC3354.4793755E-420.5986168
GTCCCAG2800.003840648820.5410121
TAGAGTG3955.7919693E-520.3813325
GTGTATG6251.3673343E-820.245221
ACTGTCC6901.9590516E-920.0015548
TACACCG8653.6379788E-1219.9437475
CATACTG8254.3655746E-1119.5166685
CAGGACT5351.4011439E-619.34734
GTATACA7753.965397E-1019.2952981