FastQCFastQC Report
Thu 23 Jun 2016
MG391777G_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391777G_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832799
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG9950.025.4273745
TACACCG7800.022.1155915
GTATAGA9150.021.3663221
CAGGTCT3854.756706E-520.9092884
GTACACC6059.43146E-920.9092884
GTACCCT2750.003464884120.9092866
GTATACA7402.1464075E-1020.2028921
CTTACAC6301.502849E-820.0795543
GTACTAC3506.0206966E-419.7144723
CTGTGCG2950.005202141619.4917099
CTATAAC8304.7293724E-1119.3977743
GTGGGCA3000.005732805519.166859
CCTAGAG4301.11257774E-418.7211063
TACCCTG3150.007597539618.254147
CTATACT7606.511982E-918.1580664
GTTATAC8259.1677066E-1018.1213823
GTGCTAC4551.7152302E-417.6924741
CATACAC7206.9432645E-817.5696093
TGTATAC6655.8280784E-717.2933963
ACACCGA8001.2283635E-817.2501626