FastQCFastQC Report
Thu 23 Jun 2016
MG391770J_S11_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391770J_S11_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348794
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG8250.022.0833325
TATACTG16500.020.7031255
GTATACA11650.020.528461
GCCCTAA5109.740179E-720.097191
GTGGGTG4608.989917E-619.8029889
GTATAGA13900.018.8441281
TACACTG11550.018.7313985
CCCCTAG3658.530482E-418.7206691
TATACAG17650.018.7090575
GTAGTAC5502.0104635E-618.6328123
CTATACT13550.018.4876614
ACCGTGC5552.1921605E-618.4649498
CTAGGAC6353.9901533E-717.931843
GTCCTAT7005.6332283E-817.8960691
CTACACC17550.017.5180284
GTATAGT10505.456968E-1217.3537641
CTTACAC9853.45608E-1117.3401813
TATAGAC11850.017.2962823
TAAGGGT6004.612324E-617.0800784
CTATACA13350.017.0587564