FastQCFastQC Report
Thu 23 Jun 2016
MG391770J_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391770J_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348794
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC9450.022.8967193
TATACTG15650.021.8302355
TAGAGCT8453.6379788E-1220.2155724
GTCTAGG5251.2864384E-619.5224651
TACACTG10600.019.3382935
CTCTAGG7103.1650416E-919.2475031
CTAGGAC4751.18466E-519.1799683
CCTAAGG4851.4177813E-518.7845043
GGACCGT5702.824927E-617.9812186
CTATACT13900.017.2050514
TTAGAGT8702.1209416E-917.0167084
TAGACTC5554.5149784E-516.4152875
GTCCATA17350.016.4093721
GTATATA14850.016.1043911
TAGAGTG5756.112222E-515.844325
TTATACT20850.015.839574
ACTGTGC6509.857875E-615.7681468
GCACCGG4400.002980406915.5292346
GTGTACG7452.1152373E-615.2860471
CCCTATA6008.790496E-515.1841392