FastQCFastQC Report
Thu 23 Jun 2016
MG391498T_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391498T_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181674
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG2455.8006273E-527.8470785
CCGCGAG1700.00900176926.7550379
CTAGGGT2250.001152473425.2686463
AGGGTGT4251.7079765E-724.0795356
GACCGTC4751.8886567E-823.9387197
TAGGACC4002.7112055E-622.7417834
AAGCCCG4103.3593296E-622.1871055
AGCCCGA2600.002672790321.8670986
ACCGTCC5255.455331E-821.658848
GTATATA14800.021.5152281
GTATAGA11950.020.9365161
TCTATAC11000.020.6743473
GTCCAGG3354.8338584E-420.368361
TTAAGGT6751.718945E-920.2149184
GTATAGG8553.6379788E-1219.9514661
TAGACCG5151.0852473E-619.871465
GGGTGTA4201.01339494E-418.9514857
TATACTG15150.018.7638475
CCAGTAC8507.8216544E-1118.7285273
TATATAC14650.018.628083