FastQCFastQC Report
Thu 23 Jun 2016
MG391498T_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG391498T_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181674
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGA1950.002189612735.717857235
TACACTG8150.025.1096345
TATATAC17150.023.2022133
GTGTAAG8500.022.7381671
CCTATAC7650.022.2923223
TATAGGC5705.4205884E-921.9403382
CCTACCC2700.003330032621.053863
TAGGCTG7752.1827873E-1120.53775
CCCTATA5109.898049E-720.063092
ATAGGGT4350.004463644720.014317235
TATACAG15950.019.9582675
TCCCCGC2850.004554562719.9457638
GTATATA14250.019.945761
CTTAAGG5151.0869026E-619.8683013
TATACTG15500.019.8042135
GTATAGG6902.2646418E-919.772321
GGACCGT4159.2552684E-519.1767676
CTCTAGA9501.8189894E-1219.147931
ACTGTGT6552.6828275E-819.0931198
CTACACT7855.511538E-1018.8277844