FastQCFastQC Report
Thu 23 Jun 2016
MG390984V_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390984V_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058056
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCC2107.407477E-427.2560024
CTATACT10550.027.1268274
CTAGGAC3801.6370905E-624.1000443
TAGTACC4852.1933374E-823.6031364
TAGACCG4452.494853E-723.152295
GTAGACC4553.0911542E-722.6434484
CTAGACT6205.475158E-1022.1564924
TATAGTG9350.022.0380085
GTATATA13150.021.7684941
TCTAGAC5856.7539077E-921.5252553
GTATAAG10850.021.106411
TATACAG14900.019.975545
GTGTAGG8653.6379788E-1219.8558851
TATACTG13550.019.4312175
GTATAGG7754.2200554E-1019.2068331
TAGAGTG4209.623621E-519.0792035
GTCTAGG4251.05193714E-418.8591981
CTTATAG7955.9480953E-1018.719223
TCAGTAC10600.018.3592343
CACCGTG4401.3748412E-418.2119667