FastQCFastQC Report
Thu 23 Jun 2016
MG390984V_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390984V_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058056
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACC1700.00872297926.9279584
ATAGGAC5555.456968E-1226.8066583
GTCTTAG8200.026.5174671
GTAGGGT2209.73523E-426.0099584
ATAGGCC3301.5017749E-524.2759593
CCGTGTG3301.5017749E-524.2759599
CCAGGAC2901.7462534E-423.678033
ACCGTGT5604.092726E-922.4800348
GTCCTAG4153.5284156E-622.0614591
GCGTGCG2600.002574497822.0084259
GCCTTAC5255.0946255E-821.798821
GTCTAGA5806.1372702E-921.704861
AGAGCTC2700.003204770221.1932988
GTCTATA7601.4551915E-1121.0817551
ACAGTGC5508.3307896E-820.8079668
CTAGACC6201.3231329E-820.3045484
GTATAGA11150.019.5016351
TAGACAG7754.2564352E-1019.1970275
TAGGACC3000.005896991619.073974
TAGGACT6602.7130227E-819.0739694