FastQCFastQC Report
Thu 23 Jun 2016
MG390982M_S2_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390982M_S2_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779452
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA7500.024.4532284
GTATGGG5701.8735591E-1024.1361221
GTCTAAG4402.2035056E-723.4504341
CATACAC6205.3842086E-1022.1853893
GTATTAT16350.021.737271
CCTATAG3253.7290482E-421.1655221
GTCATAG6601.1714292E-920.8448311
GTGTTAC5558.988718E-820.657041
AGAGTGT4456.36812E-620.6066536
CCCTATA3354.5813384E-420.5297642
CTAAGGC3354.5813384E-420.5297643
CCCTTAG2800.003915356520.4726051
TGTACAG6251.4235411E-820.1739145
GTGTAGG4557.704693E-620.157641
GTCCTAG2850.004337871420.1134361
CCTGTAC6851.8662831E-920.0802063
TAGACCC2900.00480246119.7628464
ACTGCGG3607.4395654E-419.1040848
TCCTAGA7804.5292836E-1019.1040842
GTGTAAG6152.6506495E-718.641721