FastQCFastQC Report
Thu 23 Jun 2016
MG390982M_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390982M_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779452
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGT1954.770133E-429.3848323
TATATAG6700.027.3673655
TAGCCCG1700.00866346926.9649055
ACCGGTC2608.238812E-526.4463468
CTTATAG5001.0841177E-925.2121853
GGAGTCT2400.001603352423.8751746
ACCTAGG2500.00203313922.9201681
ACAGACC4653.768837E-722.1808098
AGAGGCG2600.002553484922.0386225
CCTAGGA3153.0273324E-421.828732
CAGAGGC3153.0273324E-421.828734
CCCTATA3703.596529E-521.681241
GTGTATG6059.815267E-920.836521
TCTATAC7151.4188117E-1020.8365153
GTATAGG4456.3795105E-620.6023981
GTCCTGG2800.00392441720.4644361
CATACCG5609.922951E-820.4644365
GTATAAC8453.6379788E-1220.3433441
ACTGGCC2850.004347903720.105418
GGTTAGT3455.5939297E-419.930581