FastQCFastQC Report
Thu 23 Jun 2016
MG390975X_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390975X_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683286
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG3153.1384843E-729.117741
GTGTAGG4654.638423E-1027.1217651
ATAAGGG6451.8189894E-1224.8818683
TATACAG10800.024.4128655
TATACTG7650.023.9758075
CATAGAG2400.00160042723.8821492
ACAGGGC2901.7258018E-423.7174438
CCGTCTG3502.3358327E-522.9268659
CAGGGCA3002.171463E-422.9268659
CTAGGAC3203.3600995E-421.4939373
AGGACTG5958.069037E-921.1929025
TTAACCC3253.73109E-421.163264
TGTAGGG3304.136186E-420.8426042
GTATAGA7251.6916601E-1020.5581281
CCTGTAC4608.47322E-619.9364033
GTCTATA5301.3187055E-619.4690551
TAGGGAC2950.00529814219.4295444
CCCACTC3000.005838532519.105723
TACACCG5501.8826377E-618.7583455
CTATAGT3759.780849E-418.3414924