FastQCFastQC Report
Thu 23 Jun 2016
MG390975X_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG390975X_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683286
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTCG1006.435291E-445.8163035
CCTTAGG2101.9257077E-532.7259331
CCGTGCG1752.5369218E-432.7259339
TAAGGGT2354.152053E-529.2444524
AGACCGC2801.3677898E-424.5444496
GTCCTAT2600.0025609522.027071
TCTATAC6601.1841621E-920.8255923
ACCGTGC3455.6127546E-419.9201328
GTCCTAC4057.241383E-519.7971691
TATAGCA8204.1836756E-1119.5557382
GGGTACA2950.005323204719.4136891
GTCTAGG2950.005323204719.4136891
TAGACCG3556.8036077E-419.3590015
GTCTTAG5451.7386519E-618.914991
GTCTAAG4251.048537E-418.8655361
TCTAGAC3658.2014315E-418.8286173
CTAAGCT3708.986718E-418.5741794
CTACCCC3708.986718E-418.5741794
CTATACT7055.7098077E-817.8716094
GTGGGCC650.00865158417.849195110-114