FastQCFastQC Report
Thu 23 Jun 2016
MG386159C_S22_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG386159C_S22_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879965
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCCG1756.172133E-638.6388975
GTATAGG5750.029.4024981
TAGACCG3704.8647053E-827.412735
GTGTAGG4554.4019544E-1027.248471
ACTGTGC4501.1683369E-825.0437288
TCGATCG2450.001992985422.9993429
ATAGGGG5701.4275975E-719.7713643
GTACTAA8407.4578566E-1118.7849311
GACTGTG4851.5504147E-518.5891597
GTATTAG13300.017.796251
TATACAG5703.1188301E-617.7942285
TATACTG8901.72804E-1017.7275815
CTATAGT8301.3005774E-917.6513024
GTGTAGA5853.99117E-617.3399351
CCTTAAG7208.738789E-817.2195191
TAGGGGT6105.9510476E-616.6273924
ATACTGT11551.8189894E-1216.5874046
ACTGTAG5454.2230906E-516.5426458
ACTATAC6901.059534E-616.3328653
TATACGA4853.259821E-416.2655122