FastQCFastQC Report
Thu 23 Jun 2016
MG386159C_S22_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG386159C_S22_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879965
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGG1350.00306281433.3556256
CGGGACC2650.003163177321.240614
ATATACG3203.798922E-421.1078574
TAGACCG2800.004351709520.102725
TGGACTG5851.8982428E-719.243635
CAGACCG3700.001009775418.2554449
GTATAGG5652.896979E-617.9323371
ATACTGC7557.8143785E-917.8927546
CCTACTC3800.001207645317.7750383
AGTACTC3800.001207645317.7750385
CCTATAC7006.421578E-817.6903933
ATTATAC14300.017.3192673
GTATAAG9904.3655746E-1117.0568521
CTTACAC6606.733044E-717.0568523
TATACAT5954.744137E-617.0281875
TATATAG6005.1375337E-616.8862845
CTGAGTC5403.9386516E-516.6778139
ACCGTGC4050.001850314916.6778138
TAGTACT5454.262308E-516.5248054
TAGACAG8202.1678716E-816.4744245