FastQCFastQC Report
Thu 23 Jun 2016
MG291789G_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG291789G_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919673
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG2806.184564E-1140.8107871
AGCCCGC1600.00654369228.5613386
GTATAGG6000.026.6630481
TCCTAGG3751.4842226E-624.3723412
TAGGCCA2650.002904285221.5557274
CTATAGT4405.9448066E-620.7718834
TAGGACC3304.2336943E-420.7718834
TCTATAC10200.020.1609443
GTATACA9750.019.9240361
GTATAAA18950.019.8992981
CCGTGCA4108.120817E-519.5053049
ACGGTGC4751.1518132E-519.2413228
CCTTAGG3607.6086435E-419.0408922
GGACCTG3000.0059558319.0408926
GCCTTAG4901.5030768E-518.6563591
TATAGGG4901.5058968E-518.6523022
TATACAG12950.018.5262725
CTAGGCA3709.1472786E-418.5262724
TAGTACT6303.5438825E-718.1341824
GTCTAGG4451.5195488E-417.9750881