FastQCFastQC Report
Thu 23 Jun 2016
MG291789G_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG291789G_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919673
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCCC1601.5242284E-435.6921778
CCGAGCC2350.001446696324.3010549
CTATAGT4452.5494774E-723.0996554
TGTATAC8700.022.3178673
CCTATGG4608.751216E-619.863471
GTATATA15100.019.6661531
TAGACAG7704.0017767E-1019.2830455
TATACAG13250.018.9639935
TAAGGCT7905.638867E-1018.7948674
GTGTACG3709.163643E-418.5213451
CCTATAC5102.1298269E-517.9160733
TACACTG7106.402297E-817.6952765
TATACTG11000.017.6514025
TAAGAGC5903.8146227E-617.4226193
TATATAG10555.456968E-1217.32175
TGTACTG9302.6739144E-1017.193655
AGTGTGT4652.134714E-417.193658
ATAAGGC8001.3375029E-817.1322443
TAGACCG4050.001679797216.9207325
GTGTAGG6155.663447E-616.7143841