FastQCFastQC Report
Thu 23 Jun 2016
JG269965G_S21_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJG269965G_S21_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662082
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGG1650.00782305827.5342851
ACGGACC2250.00115964625.2397613
GTATAGA4856.1578794E-721.07651
TCTACGG2900.005063044419.5825732
TATACAG4201.0214651E-418.9298215
TGAGCCG3000.006158724418.9298215
GTATTAT13900.018.7936341
GTATATA3658.679329E-418.6705071
CTAATAC9151.0913936E-1118.6194953
TATATAG5502.0565658E-618.5856425
ATATACG3750.001040702218.1726261
GTATAAC6404.440535E-717.7467061
GTATAGC5152.428407E-517.6433281
TCTATAG4702.4160356E-416.916012
ATGTAGG4050.001743048816.8265061
GTCCGGC900.001802888116.744589215-219
ATTAGGC5554.6131514E-516.3717373
CTAGGAC4200.002222692416.2255613
GTATAAT9907.003109E-1016.0616651
CTCAGTA4300.002600681315.8482232