FastQCFastQC Report
Thu 23 Jun 2016
JG269965G_S21_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameJG269965G_S21_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662082
Sequences flagged as poor quality0
Sequence length32-241
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG1551.321494E-436.5669949
TACCGCG2450.001925857423.134227
CTATAGG4007.1282884E-519.8375931
GGACTGC2950.005652213519.2131676
TAGGACT6502.536035E-819.1836074
GTATAAA9009.094947E-1218.8929461
GTACCGC3050.006852026618.5832256
TAACACT7557.1631803E-918.0171154
GTGTAGA3850.001258004117.6661321
GTATAGG4551.9140691E-417.4396421
TACACAT4551.9140691E-417.4396425
TCTATAC4602.0808226E-417.2500823
CAGTACC7258.84811E-817.1990974
ATTATAC9904.0017767E-1117.1754063
TATACAC4100.001916943516.5889283
ACTATAC5655.465342E-516.0506463
GTATAAG7852.1883352E-715.8845151
TATAAGG11452.0008883E-1115.8403732
CTATACT6459.556126E-615.817354
CTTACAC4350.002845870615.6355413