FastQCFastQC Report
Fri 10 Jun 2016
Undetermined_S0_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814016
Sequences flagged as poor quality0
Sequence length32-241
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC19440.2388159446497366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCTAA1801.1756674E-745.171433
TGCCTAG3307.2759576E-1238.718373
TAGTTTC2256.774135E-736.1349227
GCCTAGT3004.7039066E-934.846534
AAGCCTA3753.274181E-1134.0721662
AAGACTA3801.3933459E-930.5671332
GAGTTAC1700.008115143527.3289327
ATGCCTA9400.027.1852382
GCCTAAT2658.5577834E-526.299274
CAAGACT4151.10219844E-725.1933571
CAGGAGT5653.8180588E-922.6142674
CTAGTCT2600.002362253622.336156
AAAGTAT3703.243221E-521.9739426
TAGTCTC3203.0757327E-421.7777447
CATGCAT4304.2143874E-621.612911
AGTCTCG4354.665948E-621.3605468
AGCCTAG2750.003269958521.119113
AGCCTAT2850.00402184820.3780883
CCTAATC2850.004023279520.3768375
AGGAATA4607.5679563E-620.1996485