FastQCFastQC Report
Fri 10 Jun 2016
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814016
Sequences flagged as poor quality0
Sequence length32-241
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA1309.113137E-962.4826936
TATATAC1607.166818E-1058.0196462
CCTATAG1553.6514393E-852.404849
CTATATA1801.1849261E-745.126391
ACCCTAT1952.2223321E-741.655137
CCCTATA2002.7108945E-740.613758
GTATAGG2301.780063E-840.3614921
TCTAAGC1200.001480758138.679763
TATTACA1250.001809559337.1325722
GTCTAGA1300.002193737535.7043951
ATTACAG1651.6638567E-435.1634183
CTAAGAC2051.4943371E-533.962723
ATATAGA1400.003154996833.1540831
TAGAGGT2851.1749398E-732.5724334
CCTAGAG1450.003746661132.0108382
TATACCC2602.112285E-631.2413464
TCTTGTA3609.0771755E-725.7865092
CTAGAGG2300.001163587225.2259313
TATAGGC2350.001318989824.689212
TATTTAT3451.902557E-523.54422