FastQCFastQC Report
Fri 10 Jun 2016
CIP-WT_S16_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCIP-WT_S16_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735159
Sequences flagged as poor quality0
Sequence length32-241
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG409.988245E-485.647961
TTCTAGA905.0937233E-663.438612
TAGTAAT650.006845848652.7028434
TCTATAC650.006845848652.7028433
TCTAGAG1006.5337453E-445.67583
GTTCTAG1300.002372750335.1376231
GTATTGA1954.8694058E-429.2813511
CTATTGG1600.006556313528.5493181
TATTGGG2005.6493323E-428.5473772
TGTAGTA2056.5270404E-427.8510992
TGTATAA450.001124926725.3754442
GTTCAAT2300.001277230824.8254931
GTCTATG2951.9888018E-423.2265661
TGTATCC2750.003609654320.7617283
ACCTATC4702.3195696E-417.0069472
GTGTCAT6256.6083085E-616.4444071
GGTAGAG4250.002321194616.120875
GTACCAC8052.6792077E-715.6045971
AGAGACC4400.002925466515.5712968
GTCTTGT5255.384969E-415.2263031