FastQCFastQC Report
Fri 10 Jun 2016
CIP-WT_S16_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCIP-WT_S16_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735159
Sequences flagged as poor quality0
Sequence length32-241
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAG150.0061603277151.982671
GTTTATG900.001984176572.04131235
TAAGAGA550.00355889162.174734
TAGACTG1051.28351185E-554.2795265
GTTCTAT1503.245077E-853.193941
TTATGGT650.006894068352.6093834
ATAGACT650.006894068352.6093834
AAACGTA650.006894068352.6093834
GTCTAGG903.9204903E-450.6608921
GTAAGAG1152.1988331E-549.5595663
TATATCC700.00924046248.8515742
TGTGTAA955.1187264E-447.9945342
TTACTCA1450.00408840831.444694
GCATAGA2854.961974E-627.9968091
GTCCTAT2056.595125E-427.8017081
CATAGAA1650.007687884427.6332112
TAGGAGA2209.962211E-425.9061374
TAGATCT2801.4136656E-424.4257874
TCTTGTA2450.001865027723.2626552
ATAGATC3052.5237075E-422.4236723