FastQCFastQC Report
Fri 10 Jun 2016
BB415997_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB415997_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946259
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC3306.5010863E-726.7927973
CTAGACC3501.0862314E-625.2617824
TAGACAG3651.5655223E-624.2236255
AGACCGG2300.001545822224.026156
AGACCTC2801.7436207E-423.682926
GTATAGA7057.2759576E-1221.9507711
GTATTAG13300.021.6088981
GTATATG11350.021.4258331
CCGTGCC3103.472205E-421.3910269
TAGACCG3305.2945013E-420.0945995
ACCCGTA3305.2945013E-420.0945997
TAAGCCC5551.324006E-719.9135674
CCTCTCC3406.473774E-419.503588
TATATAC9700.019.3695353
GTATAAC11150.018.836041
AGAGGGC2950.006545345818.7322526
GTCCTGG3558.645858E-418.6824471
TATACAG14200.018.6794875
GTCTCAT5352.0484258E-618.5951461
ACCGGGC3650.001043040818.1677198