FastQCFastQC Report
Fri 10 Jun 2016
BB415997_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB415997_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946259
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGGG1300.002785745534.0074467
TAAGGGT3701.7617458E-623.8971254
CCTAGAC2851.9653657E-423.2682533
GTCTAAG4752.5518602E-823.2682531
GGACCGT2902.2110736E-422.8670776
ACCTCCC2550.002815529721.6714128
GCCCAGG2650.003519180720.8536226
CTAGACT4306.4894302E-620.5626414
CCTAAGC4306.4894302E-620.5626413
CCTGGAC2700.00392168620.4674453
GTATAAG8750.020.210141
TCCTAAG4959.742616E-720.095312
GTCTAGA3957.865822E-519.5865671
TATATAC9700.019.370223
GTATGGG5151.4242323E-619.3149091
TATACTG9750.019.2708875
ACTGTGC4851.8335786E-518.2307978
GTGTATA9801.8189894E-1218.044771
GGACTGA3700.001142483117.9228446
CATAGAC3700.001142483117.9228443