FastQCFastQC Report
Fri 10 Jun 2016
BB415044X_S2_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB415044X_S2_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919715
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTCG1653.7492591E-641.547377
AAGGGTC3203.7152859E-728.5638186
GGGTCGC2956.3726293E-627.1114228
GTCCTAG3406.3055086E-726.8865181
GTATATA10050.025.0138241
CCTATAC4553.1480704E-722.5999453
CTATACT7801.8189894E-1221.9721684
TAGACAG4704.3027285E-721.878675
GTATAGA7601.4551915E-1121.0493141
GTCTTAG4405.934755E-620.7759461
CTAAGGT5007.8007906E-720.565954
GCCTCTG3354.683014E-420.4636337
GTCTCAA5058.5738793E-720.364541
GTGTATA7352.1282176E-1020.2106111
GTGCATG5301.3627614E-619.4039481
TAAGGTC3000.00595284419.0425453
GTATAAG11050.018.6137431
GTATTAG14300.018.378721
CCGTGTG3750.001000438818.2808449
CCAGGAC3150.00788857718.135763