FastQCFastQC Report
Fri 10 Jun 2016
BB415044X_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBB415044X_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919715
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAC1954.861442E-429.2905081
ACCGTGC5151.5861588E-924.3992778
CATATAC10000.023.9889283
CTAGACC3952.3344382E-623.1357944
GTATAGA7450.022.999931
CCCCCCC852.598841E-622.855574195-199
GTATAAG10050.022.7329311
GTCTAGG3052.4878196E-422.4720611
TACTCCG4153.5844623E-622.0208155
AGACTGA3703.687767E-521.6116456
GTATATA11150.020.4902211
CTACACC11150.020.4902214
GAGTAGG2800.003998708420.3987461
TAAGAGT6351.7780621E-819.788394
CCCTAGC2900.00489910519.6953412
ATAAGAG8755.456968E-1219.5827983
ACCGTAC5901.7802631E-719.3615238
CTCTTAG3607.6141837E-419.0388321
ATAGGTC3658.3541236E-418.7780258
CCAGGAC3658.3541236E-418.7780253