FastQCFastQC Report
Fri 10 Jun 2016
8304_S13_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8304_S13_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000753
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACT355.608621E-499.0370254
ATTAATA600.004760837657.77163
ATCTTAG1006.161642E-446.2242051
CTATAGA1400.003219909533.0172921
GTGTTAA1802.842206E-432.1001431
GTATTAC2404.5140543E-528.8901311
GTCTATA1600.00618815128.8901311
TCTTAGC2455.2006268E-528.2962932
ATAGATA1750.00958933426.4098744
GTATATC2751.14011236E-425.2132031
TATTGGA3351.5667345E-524.1433562
TATACCA3203.1869108E-421.664352
TCTAGAC2800.00374333520.6327132
GTGTAGA6301.4220859E-820.1772351
GTATAGC3505.8282074E-419.8103751
GTACCGT7555.664333E-918.3644836
CCTACAC11450.018.1639963
TCTTGTA3850.001107305318.0067332
CCCTCTT5850.00918384917.662031235
TTGTACA3950.001315073317.5508652