FastQCFastQC Report
Fri 10 Jun 2016
8304_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename8304_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000753
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA409.575084E-486.566012
TCATAAT852.780118E-454.315933
TAAGCTG1852.7248461E-949.9119385
GTATTAG1057.882389E-443.970041
ATATAAC1200.001520289838.4737851
ATGTTAG1350.00271018734.1989171
ATAGGGT2005.306907E-428.8553373
TAATCTG2056.131655E-428.151555
GGATATA2107.059329E-427.4812741
GTATTAC3057.631013E-626.4901471
ATATAGC2550.002188934522.6316383
TTAACGC2700.00305040821.3743253
TAACCCT3354.3733447E-420.6724825
ATACAAG2800.00376628620.6109561
ACCTTTT5050.004599326319.909958235
TGTAGTA2950.005094279519.5629432
TACGTAG2950.005094279519.5629435
TAGCACA4301.08215856E-418.7895224
TAGGAGC4050.001565854517.0994595
GGCTTAG4100.001699871416.890931