FastQCFastQC Report
Fri 10 Jun 2016
402713_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename402713_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520811
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAA450.001652740875.4483641
GTATAAG853.0643033E-453.2576751
TCTAAAG1250.002047559736.2116432
GTATAGA1601.6091466E-435.3664251
TCTATAT1300.002482039934.818893
ATATAGA1400.003567251632.3350141
TATAGAA2152.4591456E-531.5799222
GTCTTAG2253.3523705E-530.1793481
TATTGGA5657.2759576E-1226.0371322
CCCTATA2200.001039298125.7210351
GTGTAAG2200.001039298125.7210351
AATAGAC3251.4565239E-524.373222
GTCCTAT4251.7885031E-723.9659521
GGTAATA3351.840256E-523.6479971
ACCTATA2550.002455995722.1885092
GTATACA2600.002747516621.7639521
TCCTATC3804.8733513E-520.845522
GTAATAC2800.00422423120.2073922
CGTTAGT2800.00422423120.2073922
GAACATA2850.00467717919.8548321