FastQCFastQC Report
Fri 10 Jun 2016
402713_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename402713_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520811
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTAA658.15808E-569.599343
GTCTTAA550.003671637761.690331
TTATGTT1407.359888E-540.3924752
CCTTATA1250.002053115536.1916582
TGTTAGA1702.3064742E-433.2643932
TATAGGA1400.003578638232.313982
ATCTTAG1450.004249352531.1997051
TCCTATA1853.7875937E-430.5672822
TATACGA2703.4652148E-629.3219472
GTTTAGT1550.00588778729.1868211
TAGACAG2855.2780597E-627.7786875
TAATAGG1650.00798959227.4179232
TAACAGA2107.951481E-426.9283164
GTACAAA3907.837116E-826.0997541
TAGGAGT4902.7823262E-823.0814154
TAAAGAC3452.3219804E-522.947613
CTCCTAG3452.3219804E-522.947611
ATATGAG2500.002196128722.6197873
ATAACAC3002.3808987E-422.6197853
GTTAGAC2600.002757912321.7497963