FastQCFastQC Report
Fri 10 Jun 2016
402629_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename402629_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887074
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAAG303.5990615E-4110.722932
CTATAAG1102.005094E-550.3399541
GTAATAA904.5201695E-449.2212871
ATACAGT1150.001512736338.512326
GTACATA1851.0184718E-535.918241
TATACAT1300.002761227834.0685922
GTTATAG1702.6082978E-432.5729141
AATAGGA1500.00556322129.5261142
CTACAGA2355.2253785E-528.276061
TACATAC2006.766534E-427.6807332
TATAGAG1600.00762453127.6807332
TACAGAA1600.00762453127.6807332
TATGCAG3357.294657E-726.4412985
TGCATAG2250.001345576524.6050952
GTCCTAT2801.7193524E-423.7316911
AAAAAAA2055.656524E-623.480074235
GTCCTAG2450.00220569122.6016121
AATAGCA2550.002786193721.7103772
GTGCATA3254.7104998E-420.4457661
ATACATC4059.3994575E-519.1372973